1. You should already be using either Trend Micro Internet Security software or Avast software.
If you are using another Brand, then it would be in your best interest to periodically scan your computer for viruses or malware that your software misses.
Here is a list of other known good virus scanning software and malware scanning software.
1. Kaspersky - Link is here.
2. HitmanPro - can be installed along with other brands of anti-virus software. Specifically good to detect hidden Rootkits. Link is here.
3.
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You can Submit a file for review:
If you suspect that there is an infected file on the computer, there is a place where you can upload individual files for scanning. This is GREAT to scan email attachments that people send to you, i.e. PowerPoint presentations (.PPS or .PPT files)
The place is VirusTotal.Com.